%0 Journal Article
%T Isolation and characterization of Serratia marcescens phage<br>一株粘质沙雷氏菌烈性噬菌体污水分离及特性
%A Long Yu
%A Zhanbo Wen
%A Wenhui Yang
%A Na Li
%A Jie Wang
%A Jianchun Lu
%A Jingsong Li
%A <br>于龙
%A 温占波
%A 杨文慧
%A 李娜
%A 王洁
%A 鹿建春
%A 李劲松
%J 微生物学报
%D 2008
%I 
%X OBJECTIVE: To isolate the bacteriophage of Serratia marcescens (8039) from sewage, and to study on its biological characteristics. METHODS: We used Serratia marcescens (8039) as the host to isolate phage from raw sewage from the Sewage Treatment Center of Hospital. Phage SM701 was examined in electron microscope. The genome of SM701 was extracted and the size of its nucleic acid was identified with restriction enzyme analysis. Phage isolated was subjected to plaque forming unit (PFU) assay using double layer agar medium plate method and morphological properties of plaque was observed, and finally optimal MOI multiplicity of infection and one-step growth experiments were carried out. RESULTS: Phage SM701 specific to Serratia marcescens (8039) was isolated successfully from the raw sewage. SM701 had an isometric polyhedral head (about 64 nm in diameter) and a long noncontractile tail (about 143 nm long). The nucleic acid could be cut off by dsDNA restriction enzyme BamH I or HindIII and its complete size was about 57kb. The plaque of SM701 was transparent about 1mm in diameter at the 12th hour. When MOI equaled 10, the number of phage offspring was higher. One-stepgrowth kinetics was determined according to the results of one-step growth experiment, which showed that the latent period was about 30 min, the rise period was about 100 min, and the average bust size was about 63 pfu/cell. CONCLUSION: Phage SM701 belonged to tailed family: siphoviridae and lytic bacteriophage. It was quite easy to observe and count the plaques due to existing color differences between lytic and nonlytic zone.
%K Serratia marcescens
%K bacteriophage
%K isolation
%K biology and morphology<br>粘质沙雷氏菌
%K 噬菌体
%K 分离
%K 生物学
%K 形态学
%K 粘质沙雷氏菌
%K 噬菌体
%K 水分离
%K 特性
%K phage
%K characterization
%K 相关
%K 呼肠
%K 呼吸道
%K 计数
%K 差异明显
%K 颜色
%K 细菌生长
%K 分类法
%K 分类标准
%K 委员会
%K 病毒
%K 国际
%K 爆发期
%K 潜伏期
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=9DF96E2D0A29DD7B25399938C32B6D28&yid=67289AFF6305E306&vid=B6DA1AC076E37400&iid=E158A972A605785F&sid=E3C3E274D87A8C16&eid=51F9E747BA1ACB45&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=18