%0 Journal Article
%T Histopathological Research and Immunofluorescence of AVND Virus Infection in Cultured Scallop Chlamys farreri
栉孔扇贝感染急性病毒性坏死症病毒的组织病理学与免疫荧光检测
%A FU Chong-Luo
%A SONG Wei-Bo LI Yun ZHANG Yong-Zhong YAN Hua-Chao
%A
付崇罗
%A 宋微波
%A 李赟
%A 张永忠
%A 闫华超
%J 微生物学报
%D 2004
%I
%X The naturally infected scallops Chlamys farreri sampled during mass mortality in summer of 2003 was detected by means of histopathological and MAb-based immunofluorescence assay (IFA). The results of histological examination demonstrated that a series of histopathological changes including cell swelling, basophilic increase, disorder, partial sloughing and excessive sloughing were always observed in epithelia of many different organs, e.g. mantle, gills, stomach, intestine and kidney. Additionally, the infected tissues were applied for in situ detection of the "acute virus necrobiotic disease" (AVND) virus by means of specific MAb-based IFA, and the result demonstrated that this pathological changes or lesions were perfectly coincident with the positive cells (fluorescencing cells). The positive cells were denser in some local area of epithelia, and exhibited serious pathological lesions, which would reveal the roles of this virus in pathogenesis and further confirm that the AVND virus is the main causative agent of mass mortalities among cultured scallop Chlamys farreri farmed in northern coast of China.
%K Scallop
%K Chlamys farreri
%K AVND virus
%K Histopathology
%K Immunofluorescence assay
组织病理学
%K 上皮组织
%K 感染
%K 急性病毒性坏死症病毒
%K 单克隆抗体
%K 病毒
%K 免疫荧光检测
%K 栉孔扇贝
%K 大规模死亡
%K 外套膜
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=3136CE646A61BEE1&yid=D0E58B75BFD8E51C&vid=1AE5323881A5ECDC&iid=B31275AF3241DB2D&sid=6D237E9625601349&eid=195767709E606C85&journal_id=0001-6209&journal_name=微生物学报&referenced_num=5&reference_num=19