%0 Journal Article
%T Study on the structure and function of a stable methane-oxidizing mixed microbial consortium<br>含有甲烷氧化菌的混合菌群特性研究
%A LUO Ming-fang
%A WU Hao
%A WANG Lei
%A XING Xin-hui
%A <br>罗明芳
%A 吴昊
%A 王磊
%A 邢新会
%J 微生物学报
%D 2007
%I 
%X From an agricultural sample taken in Chongqing, a stable methane-oxidizing mixed microbial consortium was established by enrichment culture with methane as a sole source of carbon and energy. The mixed consortium showed high capability of phenol degradation and 1,2-epoxypropane production from propene. More than 99% of phenol at an initial concentration of 600mg/L could be degraded by the mixed microbial consortium after 11 h of cultivation. The productivity of 1, 2-epoxypropane could be increased with the decrease of phosphate concentration. The concentration of 1, 2-epoxypropane produced could reach to 5.0mmol/L. The bacterial structure of the methane-oxidizing mixed microbial consortium was analyzed by pure culture isolation combining with 16S rRNA and PCR of the related MMO functional genes. The results showed that the methane-oxidizing mixed microbial consortium was composed of a type 1U methanotroph identified as Methylosinus trichosporium and at least 4 kinds of heterotrophs ( Comamonas testosteroni, Cupriavidus metallidurans, Acinetobacter junii and Stenotrophomonas maltophilia ). M. trichosporium Y9, isolated from the mixed consortium, harbored both sMMO and pMMO genes.
%K Methylosinus trichosporium<br>苯酚
%K 环氧丙烷
%K 甲烷氧化菌
%K 混合菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=361F73232E2C158E&yid=A732AF04DDA03BB3&vid=F4B561950EE1D31A&iid=CA4FD0336C81A37A&sid=89F76E117E9BDB76&eid=91C9056D8E8856E0&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=16