%0 Journal Article
%T Isolation and identification of two xylanase-producing extremely alkali-tolerant strains of Bacillus halodurans from Turpan in China<br>中国吐鲁番两株产木聚糖酶的极端耐碱Bacillus halodurans的分类鉴定
%A YI Xia
%A XIE Zhou-jie
%A DENG Ai-hua
%A WANG Ning
%A Erkin Rahman
%A <br>易霞
%A 谢周杰
%A 邓爱华
%A 王宁
%A 艾尔肯·热合曼
%J 微生物学报
%D 2006
%I 
%X Bacillus halodurans XJU-1 and XJU-80 were characterized in terms of physiological and biochemical characteristics, and 16S rDNA sequence homology and DNA-DNA hybridization analysis. The two isolates can grow in nutrient broth at a broad range of pH values from 4.5 to 12.6 for XJU-1 and from 3.8 to 12.8 for XJU-80, respectively. And the optimum temperature of growth were around 39 degrees C and 42 degrees C, respectively. Phylogenetic analysis of the two strains based on comparison of 16S rRNA sequence revealed that they are closely related to Bacillus halodurans C-125 and DSM497Twith 99% identity. DNA-DNA hybridization showed that the highest levels of DNA-DNA relatedness were found between the two strains (85%) and the B. halodurans type strains (81.3% and 71.5%), respectively. Moreover, the G + C content of the genomic DNA was 40.5 mol% for XJU-1 and 42.2 mol% for XJU-80.Our results demonstrate that strains XJU-1 and XJU-80 should be classified as two new members of the species B. halodurans.
%K Bacillus halodurans
%K G C content
%K 16S rRNA
%K DNA_DNA hybridization
%K Phylogenetic analysis CLC number: Q936 Document code: A Article ID: 0001_6209(2006)06_0951_05<br>Bacillus
%K halodurans
%K G
%K C
%K mol%含量
%K 16S
%K rRNA
%K DNA-DNA同源杂交
%K 进化树分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=217428519BE89085&yid=37904DC365DD7266&vid=D997634CFE9B6321&iid=B31275AF3241DB2D&sid=9822743C2D2BE348&eid=2F26E27A20AEB31F&journal_id=0001-6209&journal_name=微生物学报&referenced_num=2&reference_num=28