%0 Journal Article
%T Demonstration of Simultaneous Infection with Dengue Type 2 and 3 in A Chinese Patient<br>国内首次自患者标本中同时分离的登革2型和3型病毒的鉴定
%A Man Yu
%A Wen-Ming Peng
%A Bao-Chang Fan
%A Yong-Qiang Deng
%A E-De Qin
%A <br>于曼
%A 彭文明
%A 范宝昌
%A 邓永强
%A 秦鄂德
%J 微生物学报
%D 2004
%I 
%X IgG and IgM antibodies to dengue virus(DEN) in patient serum sample were detected with an indirect immuno- fluorescence assay. The patient's serum was inoculated into the culture of C6/36 cells to isolate dengue virus. Viral RNA was extracted from cultured supernatant and RT-PCR amplification and sequencing were carried out. The result showed that there were anti-dengue virus IgG and IgM antibodies in the serum. Both dengue type 2 and 3 virus specific sequences were confirmed by RT-PCR and sequence analysis. The results presented here demonstrated the simultaneous infection with dengue type 2 and 3 viruses in the Chinese patient.
%K Dengue fever
%K Dengue virus
%K Mixed infection
%K Antibody<br>登革热
%K 登革病毒
%K 混合感染
%K 抗体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=9D27E1FA6E5A92E8&yid=D0E58B75BFD8E51C&vid=1AE5323881A5ECDC&iid=B31275AF3241DB2D&sid=E5ED9059DE792E50&eid=2A92ABD90588B251&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=7