%0 Journal Article
%T Recombinant cIL-18-binding protein as an antagonist to cIL-18 enhanced PBMCs secreting IFN-γ<br>重组IL-18BP拮抗重组鸡IL-18刺激外周血单核细胞产生IFN-γ的作用
%A Wenqiang Liu
%A Guiqin Liu
%A Xuling Qin
%A Guiying Wang
%A <br>刘文强
%A 刘桂芹
%A 秦绪岭
%A 王桂英
%J 微生物学报
%D 2010
%I 
%X Objective]Interleukin-18(IL-18) is a proinflammatory cytokines that plays a key role of immune regulation through activating the Th 1 cell and NK cell secreting interferon-γ(IFN-γ) .IL-18-binding proteins(IL-18BP) secreted in human and mouse can antagonise the activity of IL-18.The homologue gene of IL-18BP from fowlpox virus genome was predicted and identified of its expressed protein, to use as an antagonist to IL-18 guiding disease.Methods]The cIL-18BP gene was isolated from the genome of fowlpox vaccine virus by PCR through a pair of special primers and cloned into vectors pPICZαA, then expressed in yeast GS115.The biologic activity of recombinant proteins binding with cIL-18 was measured, inhibiting the activity of cIL-18 enhancing relative cells secreting IFN-γ was confirmed through ELISA.Results]The cIL-18BP gene was cloned from fowlpox virus and acquired high performance expression in yeast systems induced with methanol identified by SDS-PAGE.Recombinant cIL-18BP purified can bind specifically with recombinant cIL-18 determined by ELISA test.cIL-18BP can antagonise the activity of cIL-18 with determining the IFN-γ concentration secreting in PBMCs and MSB1 cells stimulated by cIL-18 respectively.Conclusion]The experiment indicates that recombinant cIL-18BP can inhibit the activity of cIL-18 stimulating related immune cells secreting IFN-γ.Deletion of the cIL-18BP from virus may improve the safety and immunogenicity of fowlpox virus vaccine.
%K Keywords: fowlpox virus
%K IL-18BP
%K activity<br>关键词：鸡痘病毒
%K IL-18结合蛋白
%K 活性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=564D211F8E60C1F944A51DA57776FDC9&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=E158A972A605785F&sid=60E9AACF61B3107F&eid=B99A53AADE50D922&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0