%0 Journal Article
%T Characterization of Murine Leukemia Virus Recombinants Bearing prrsv GP5 Glycoproteins<br>整合猪繁殖与呼吸综合征病毒GP5蛋白的重组鼠白血病病毒特性
%A 党占国
%A 夏平安
%A 周斌
%A 尹彦涛
%A 王建举
%A 柴春霞
%A 崔保安
%A 陈溥言
%J 微生物学报
%D 2008
%I 
%X The highly virulent PRRSV isolate strain HN-1/06 was cultivated on Marc-145. To study the viral entry mechanisms, the GP5 gene of PRRSV isolate was amplified by RT-PCR and cloned into pcDNA3.0 to generate the expressing plasmid pcDNA-GP5. pcDNA-GP5 was transfected into 293T by the calcium phosphate precipitation method. Analysis of flow cytometry confirmed that the GP5 proteins were expressed in surface of the 293T cells. Then 293T cells were transfected with pcDNA-GP5, pHIT60 and pHIT111 plasmids to generate pseudotyping virus. The pseudotyping virus supernatant was harvested 48 hours post-transfection and was detected by Western blotting and infection assay. Western blotting indicated that the GP5 glycoproteins were incorporated into the retroviral pseudotyped virus. Infection assay showed that the pseudotyped virus infected 293T and Mark-145 cell. The pseudotyped virus could be used to further study infectious mechanism of PRRSV.
%K murine leukemia virus
%K prrsv
%K GP5 gene
%K pseudotypes<br>鼠白血病病毒
%K 猪繁殖与呼吸综合征病毒
%K GP5基因
%K 假病毒
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=12D6FFF56D325ED77BA3811EF14A4F78&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=94C357A881DFC066&sid=5E191A234CD3698F&eid=A1BB529A18D3A83E&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0