%0 Journal Article
%T Expression and Characterization of The Recombination Anthrax Lethal Factor<br>重组炭疽致死因子的表达及生物活性分析
%A GUO Qiang XU Jun-Jie DONG Da-Yong FU Ling CHEN Wei
%A <br>郭强
%A 徐俊杰
%A 董大勇
%A 付玲
%A 陈薇
%A 宋小红
%J 微生物学报
%D 2004
%I 
%X The gene encoding anthrax lehtal factor (LF) was cloned into a secretory expression plasmid and then expressed in periplasmic space of E.coli. The recombinant LF (rLF) expressed was about 4% of the total proteins in E.coli. About 3 mg electrophoresis purity rLF could be obtained after the purification of 1 liter culure using ion exchange chromatography and gel filtration. The result of sequencing assay showed that the N-terminal amino acid sequence of rLF was identical to the N-terminal sequence of natural LF. In vitro toxicity analysis also shows that rLF has an excellent biological activity. The successful expression of rLF has placed a solid foundation for the research on toxicity mechanism of LF, developing new anthrax vaccines, and screening for inhibitors against anthrax toxin.
%K Bacillus anthracis
%K lethal factor
%K protein expression
%K protein purification
%K Escherichia coli<br>炭疽杆菌
%K 致死因子
%K 蛋白表达
%K 蛋白纯化
%K 大肠杆菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=A0BB58055B7970D2&yid=D0E58B75BFD8E51C&vid=1AE5323881A5ECDC&iid=B31275AF3241DB2D&sid=762CFFBBDED11937&eid=8A6D8366EA57F0FF&journal_id=0001-6209&journal_name=微生物学报&referenced_num=11&reference_num=8