%0 Journal Article
%T Classification and identification of Vibrio cholerae and Vibrio parahaemolyticus isolates based on gyrB gene phylogenetic analysis<br>霍乱弧菌和副溶血弧菌分离株的gyrB基因系统发育分析
%A HOU Xiao-li
%A CAO Qing-yi
%A PAN Jin-cao
%A CHEN Zhi
%A <br>侯晓丽
%A 曹清毅
%A 潘劲草
%A 陈智
%J 微生物学报
%D 2006
%I 
%X In order to validate the usefulness of gyrB genotype for the classification and identification of Vibrio cholerae and Vibrio parahaemolyticus isolates, the phylogenetic analysis of 13 V. cholerae, 8 V. parahaemolyticus, 2 Aeromonas hydrophila and 1 Plesiomonas shigelloides strains was carried out using the partial coding sequence of gyrB, a gene that encodes the B subunit of DNA gyrase (topoisomerase type II ) in bacteria. These strains were separately clustered at species level and typed by the DNA sequences of reference strains from GenBank. CtxA positive V. cholerae strains including 8 clincical isolates of 0139 and 2 clinical isolates of 01 formed one cluster. Four V. parahaemolyticus strains of 1 isolate from 2002 Zhejiang outbreak patient ( tdh positive), 2 clinical isoltates from 2004 and 1 strain from Japan were grouped with an environmental isolate ( trh positive) from 2001. GyrB genotype is applicable to species identification of V. cholerae, V. parahaemolyticus, A. hydrophila and P. shigelloides isolates. The ctxA positive 0139 and 01 group of V. cholerae are closely related, as reflected by gyrB sequence divergence. Furthermore, the toxigenic V. parahaemolyticus strain isolated from environments may be the potential pathogen to the local prevalent and sporadic cases.
%K gyrB gene
%K Phylogenetic analysis
%K Vibrio cholerae
%K Vibrio parahaemolyticus
%K Aeromonas hydrophila
%K Plesiomonas shigelloides<br>gyrB基因
%K 系统发育分析
%K 霍乱弧菌
%K 副溶血弧菌
%K 嗜水气单胞菌
%K 类志贺邻单胞菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=565F81FE9ABA69DC&yid=37904DC365DD7266&vid=D997634CFE9B6321&iid=B31275AF3241DB2D&sid=BEBF2238C7F1C1F1&eid=BF6EDE6C10074464&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=19