%0 Journal Article
%T The expression of gene related to salt tolerance from Sinorhizobium meliloti 042BM in Escherichia coli and purification of its fusion protein<br>苜蓿中华根瘤菌与耐盐有关基因rstA在大肠杆菌中的高效表达及其产物的纯化
%A GE Shi-Chao
%A WANG Lei
%A LI Xiao-hong
%A QI Su-wei
%A YANG Su-sheng
%A <br>葛世超
%A 王磊
%A 李小红
%A 亓苏伟
%A 杨苏声
%J 微生物学报
%D 2005
%I 
%X A 1.9kb DNA fragment related to salt tolerance ofS.meliloti strain 042BM containing two open reading frames were obtained by PCR amplication and ligated into shuttle vector pBBR1-MCS2. The complementation experiment showed that ORF2 is related to salt tolerance and named asrstA gene. Then the gene was cloned into the expression vector pThio-HisA, B and C, respectively, and recombinant expression vectors pGSA, pGB and pGC were constructed,and transformed intoE.coli Top10. Inducing by IPTG and analyzing with SDS-PAGE, the fusion protein encoded by pGSA was obtained,and it is 36% content of whole cell protein. It was isolated and purified by affinity chromography on ProBond TM , and the inclusion body precipitated by saturated sulfate ammonium, and 95% purity of fusion protein was obtained. The final product displayed a single band with a corresponding molecular weight 43kD in SDS-PAGE, and was verified by the Western blot.
%K Sinorhizobium meliloti
%K Salt tolerance
%K Expression
%K Purification<br>苜蓿中华根瘤菌
%K 耐盐
%K 表达
%K 纯化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=CE5C20D97599788F&yid=2DD7160C83D0ACED&vid=94E7F66E6C42FA23&iid=38B194292C032A66&sid=366B1248A15658C5&eid=D9202C57BAB9096F&journal_id=0001-6209&journal_name=微生物学报&referenced_num=1&reference_num=11