%0 Journal Article
%T Determination of fibronectin-binding region of FBPS of Streptococcus suis type 2<br>猪链球菌2型FBPS的纤连蛋白结合部位的初步确定
%A SUN Li-yun
%A FAN Hong-jie
%A LU Cheng-ping
%A <br>孙理云
%A 范红结
%A 陆承平
%J 微生物学报
%D 2005
%I 
%X Fusion expression plasmid pFBPS, pFBPS (7 - 82), pFBPS (7 - 165) and pFBPS3 (87 - 320) of Streptococcus suis type strain HA9801 were generated by cloning different fragments of fbps amplified from pMD-T-FBPS by PCR into plasmid pET-32a(+). It has been confirmed that the recombinant proteins, FBPS, FBPS(87 -320), and FBPS(7 - 167), which are expressed by recombinant plasmid pFBPS, pFBPS(87 - 320) and pFBPS(7 - 165), respectively, bound human fibronectin by ligand affinity Western blot assay. The results indicate the primary fibronectin-binding domain of FBPS lies within 87 - 165 amino acid residues region.
%K Streptococcus suis type 2
%K Fibronectin/fibrinogen-binding protein
%K Fusion expression
%K Fibronect-binding domain<br>猪链球菌2型
%K 纤连蛋白/血纤蛋白原结合蛋白
%K 融合表达
%K 纤连蛋白结合部位
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=7D7131AD8BCC0940&yid=2DD7160C83D0ACED&vid=94E7F66E6C42FA23&iid=94C357A881DFC066&sid=04CF423CEE45E258&eid=895BB10EB8F3BD49&journal_id=0001-6209&journal_name=微生物学报&referenced_num=2&reference_num=19