%0 Journal Article
%T Expression of bovine interferon gamma in recombinant baculovirus and determination of its antiviral activity<br>牛γ-干扰素在重组杆状病毒中的表达及其抗病毒活性的测定
%A QIN Li-ting
%A WANG Xi-jun
%A Hu Sen
%A LIU Si-dang
%A BU Zhi-gao
%A <br>秦立廷
%A 王喜军
%A 胡森
%A 刘思当
%A 步志高
%J 微生物学报
%D 2007
%I 
%X The full-length bovine interferon gamma (BoIFN-gamma) cDNA, including the secretion signal peptide coding region was recloned into baculovirus honor vectors pFastBac 1 of Bac-To-Bac system. These recombinant plasmids, pFastBac 1-BoIFN-gamma, were transformed into DH(10Bac) host bacteria to get recombinant shuttle plasmids, rBacmid-BoIFN-gamma. Recombinant baculovirus, rBac-BoIFN-gamma, was generated for expressing BoIFN-gamma, by transfecting recombinant Bacmid-BoIFN-gamma with Cellfectin Reagen into sf9 insect cells. BoIFN-gamma efficiently expressed by recombinant baculovirus in sf9 cells was testified by indirect immunofluorescence assay and indirect ELISA with monoclonal antibody against Bovine interferon-gamma. Furthermore, VSV * GFP, recombinant Vesicular Stomatitis Virus expressing green fluorescence protein and MDBK were used to determine the anti-viral activity of rBoIFN-gamma. The result shows rBoIFN-gamma could inhibit the replication of the VSV * GFP in MDBK cells and the antiviral activity of supernatant was 2 x 10(5) IU/mL. The antiviral activity of rBoIFN-gamma could be blocked by anti-BoIFN-gamma mouse serum. The results demonstrated that the recombinant baculovirus could express BoIFN-gamma efficiently and rBoIFN-gamma had high antiviral activity.
%K Interferon gamma
%K bovine
%K recombinant baculovirus
%K antiviral activity<br>γ-干扰素
%K 牛
%K 重组杆状病毒
%K 抗病毒活性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=F01CEEAC27E5C255&yid=A732AF04DDA03BB3&vid=F4B561950EE1D31A&iid=38B194292C032A66&sid=9296A146D1D94BC4&eid=B7B25E832E7F23D8&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=13