%0 Journal Article
%T Glutathione plays an anti-oxidant role in Lactococcus lactis<br>谷胱甘肽在乳酸乳球菌抵抗氧胁迫中的保护作用
%A FU Rui-yan
%A CHEN Jian
%A LI Yin
%A <br>傅瑞燕
%A 陈坚
%A 李寅
%J 微生物学报
%D 2006
%I 
%X To assess the physiological function of GSH in resistance to oxidative stress in Lactococcus lactis ssp.cremoris NZ9000,the recombinant strain NZ9000(pNZ3203) capable of producing GSH was used as the experiment materials.The anti-oxidant role of glutathione was observed under higher H_2O_2 dosage,i.e.,150 mmol/L H_2O_2 treatment for 15 min.The resistance of NZ9000(pNZ3203) cells grown for 3 h,5 h,and 7 h(nisin-induced for 1 h,3 h and 5 h) were 1.8-fold,2.6-fold,and 2.9-fold that of NZ9000(pNZ8148) cells,respectively.In addition,the survival of NZ9000(pNZ3203) cells grown for 5 h(nisin-induced for 3 h),upon treatment of 20 mmol/L menadione for 60 min,was 6.2-fold that of NZ9000(pN8148) cells.Therefore,introduction a new biosynthetic pathway of glutathione could confer higher resistance to oxidative stress on L.lactis NZ9000.
%K Glutathione
%K Lactococcus lactis
%K Oxidative stress<br>GSH
%K 乳酸乳球菌
%K 氧胁迫
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=431A3D4C4E34BCA1&yid=37904DC365DD7266&vid=D997634CFE9B6321&iid=38B194292C032A66&sid=9EF602EA28138BEA&eid=05340B75C67FF664&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=26