%0 Journal Article
%T Construction and pathogenic identification of aes-31 gene mutant of Avian Pathogenic Escherichia coli strain E058<br>禽病原性大肠杆菌aes-31基因突变株的构建和致病性鉴定
%A Haixia Huan
%A Ke Zhang
%A Xiang Chen
%A Song Gao
%A Xiufan Liu
%A <br>宦海霞
%A 张科
%A 陈祥
%A 高崧
%A 刘秀梵
%J 微生物学报
%D 2010
%I 
%X Abstract: Objective] To find the primary function of aes-31 fragment through construction of defined mutation of Avian Pathogenic Escherichia coli strain E058 and animal experiments. Methods] The fragment of aes-31 was generated by PCR and cloned into pGEM-T-easy vector. A resultant suicide vector containing the aes-31 fragment named pMEG375-aes-31 was constructed and transformed to a receptor strain SM10. Then recombinant strain SM10 was hybridized with E058 strain in solid state. Mutant derivatives of strain E058 were generated by homologous recombination and were named E058(?aes-31). The 50% lethal dose (LD50) of E058 and E058(?aes-31) in commercial day-old chickens experimentally inoculated via intratrachea were 104.3CFU and 103.5CFU, respectively. The same way was used to inoculate with 108 CFU to obtain the pathogenic ability of E058 and E058(?aes-31) in 35-days-old SPF chickens. In the chicken challenge model, the mutant was tested to determine the individual function for virulence and persistence in 2-week-old SPF chicks. Results] The pathogenicity test for E058 strain and E058(?aes-31) strain showed that the mutant had a higher mortality (75%) to 35-day-old specific pathogen-free (SPF) chicks than that of E058 (62.5%). In the chicken challenge model, there was no obviously CFUs difference in blood and lung in chicks of E058 group and E058(?aes-31) group 6 hours after inoculation. After 24 hours there was obvious CFUs difference in heart, liver, spleen, lung and blood in chicks of E058 group and E058(?aes-31) group. After 48 hours, there was also obvious CFUs difference in heart, liver and spleen in chicks of E058 group and E058(?aes-31) group E058(?aes-31) had a trend of increasing virulence in chicks. Conclusion] Aes-31 might be associated with negative regulatory gene for E058 virulence and its actual function needed further study.
%K Keywords: APEC E058 strain
%K aes-31
%K mutant
%K pathogenic identification<br>关键词：禽病原性大肠杆菌E058株
%K aes-31基因片段
%K 突变株
%K 致病性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=A8DB8FDE66F7A839E9F4B2073BFACF39&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=59906B3B2830C2C5&sid=B219870B99929345&eid=380BA99738E9DB23&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0