%0 Journal Article
%T Effects of signal peptide sequence of hemagglutinin on the expression of green fluorescent protein and hemagglutinin fusion gene<br>信号肽序列对禽流感病毒H5N1 HA 蛋白GFP融合分子在真核细胞中表达的影响
%A Yan Su
%A Baojiang Zhang
%A Yu Shen
%A Jiasi Huang
%A <br>苏艳
%A 张宝江
%A 申煜
%A 黄嘉驷
%J 微生物学报
%D 2009
%I 
%X Abstract: Objective] Purified avian influenza virus (AIV) hemagglutinin (HA) gene fragment was inserted into green fluorescent protein (GFP) expression vector, pEGFP-C1. The role of signal peptide in the HA-GFP expression in 293T cells was investigated by cutting the signal peptide or placing it in different locations of HA-GFP fusion gene. Methods] The expression of GFP in 293T cells was examined directly with fluorescence microscope and flow cytometry analysis. Results] After transfected with pEGFP-C1, M1, M2 and M3 plasmid, under fluorescent microscope the HA-GFP fusion protein was successfully expressed. Fluorescence was seen homogeneously distributed in the entire cell body of the cells transfected by the empty vector pEGFP-C1, whereas signal peptide could obviously reduced the expression of GFP-HA compared with recombinant plasmid without signal peptide. In addition, we found that there was no significant effect of the location of signal peptide on the expression of HA-GFP fusion protein.
%K 禽流感病毒HA蛋白
%K 绿色荧光蛋白
%K 融合分子
%K 真核表达
%K 信号肽序列
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=3C4078CB14497943372B8977D77B7E2B&yid=DE12191FBD62783C&vid=2A3781E88AB1776F&iid=38B194292C032A66&sid=381FB4265090A8E0&eid=35E8A259891FB32F&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=23