%0 Journal Article
%T Cross Antigenicity Test of Iron-regulated Outer Membrane Proteins (IROMPs) of Leporid Pasteurella multocida<br>兔多杀性巴氏杆菌铁调节外膜蛋白（IROMPs）抗原交叉性试验
%A GU Hong-Wei LU Cheng-Ping~
%A <br>顾宏伟
%A 陆承平
%J 微生物学报
%D 2004
%I 
%X Leporid Pasteurella multocida strain JS, C51-2, C51-3 and C51-17 were cultured in iron-replete, iron-restricted and normal conditions respectively. The bacterium cells grown under above conditions were measured to characterize iron-regulated outer membrane proteins (IROMPs) by the Congo Red binding test, all tested strains showed greater binding to Congo Red in case of the expression of IROMPs. The SDS-PAGE patterns of OMP extracts of these four strains grown under the iron-rich, iron-restricted or normal conditions, demonstrated that the IROMPs of 147kD,135kD,99kD,94kD,82kD and 72kD were expressed when grown in iron-restricted media, other than in iron-replete and normal conditions. Murine serum antibodies against the whole-cell bacterin of strain C51-17 grown in normal condition, couldn't react with the IROMPs. The IROMP extracts from strain C51-17 could elicit the hosts to produce antibodies, and antibodies could react with the IROMPs of the four strains by western blots. The bands of 99kD, 94kD or 87.6kD IROMP had the common antigens among the four strains in higher titers by ELISA. It showed that the IROMP extracts from P. multocida grown in iron-restricted condition, maybe had the epitopes of cross-protected factors (CPFs).
%K Pasteurella multocida
%K Rabbit
%K IROMPs
%K Cross Antigenicity<br>兔多杀性巴氏杆菌
%K 铁调节外膜蛋白
%K 交叉抗原性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=1D5BE31A00207362&yid=D0E58B75BFD8E51C&vid=1AE5323881A5ECDC&iid=94C357A881DFC066&sid=06DAE5E1DF7D0B6A&eid=93661EC4C0CCEA67&journal_id=0001-6209&journal_name=微生物学报&referenced_num=4&reference_num=14