%0 Journal Article
%T Studies on the isolation and biological characterization of actinophages infecting Micromonospora sp. 40027<br>小单孢菌40027菌株噬菌体的分离及其生物学特性的研究
%A LI Xiao-hua
%A ZHOU Xiu-fen
%A DENG Zi-xin
%A <br>李晓华
%A 周秀芬
%A 邓子新
%J 微生物学报
%D 2007
%I 
%X Three actinophages, phiHAU7, phiHAU9 and phiHAU11, were isolated from soil using Micromonospora sp. 40027 as a indicator strain. Three phages showed very narrow host-ranges. Three phages could infect both Micromonospora sp. 40027 and Micromonospora A-M-01, phiHAU9 and phiHAU11 could also form plaques on Micromonospora purprea. All of the three phage particles have the hexagonal heads and tails; the plaque formation of three phages on Micromonospora sp. 40027 were best on DNA medium with addition of 32 mM Ca2+ and 30 mM Mg2+; phiHAU7 was stable at pH 6-12, and other phages were stable at pH 6-10; the suitable incubation temperature for the propagation of three phages was between 28 degrees C - 37 degrees C; 53% of phiHAU7 remained viable and none of other phages was alive when they were incubated at 60 degrees C for 30 min. Restriction digestion analysis of genomes of three phages indicated that they were all double-stranded DNA with cohesive ends, their genome size are ca. 60 kb, 58 kb and 55 kb, respectively.
%K Micromonospora sp
%K 40027
%K Actinophages
%K Fortimicin A<br>小单孢菌40027菌株
%K 噬菌体
%K 福堤霉素A
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=D1D493869361AF50&yid=A732AF04DDA03BB3&vid=F4B561950EE1D31A&iid=38B194292C032A66&sid=BE5DBC360CD4FFB9&eid=5CB576B96D187F64&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=11