%0 Journal Article
%T CLONING AND SEQUENCING OF 16S RRNA GENE OF PHYTOPLASMA CWB1 STRAIN ASSOCIATED WITH CACTUS WITCHES''''BROOM<br>仙人掌丛枝病植原体CWB1株系16SrRNA基因克隆及序列分析
%A Cai Hong Li Fan Kong Baohua Chen Hairu
%A <br>蔡红
%A 李凡
%A 孔宝华
%A 陈海如
%J 微生物学报
%D 2001
%I 
%X A 1.5 kb DNA fragment was amplified in DNA samples extracted from Opuntia salmiana porm showed witches'-broom symptom. The result indicates the existence of phytoplasma associated with this disease and this phytoplasma was designated as CWB1. The amplified fragment was ligated to pGEM-T easy vector and then transformed into JM109 strain of E. coli. Cloned DNA fragments were verified by PCR, restriction endonuclease (EcoRI) digestion and sequence analysis. The result revealed that the 16S rRNA gene of CWB1 consists of 1489 bp and shared 99.7% homology with Faba bean phyllody which belongs to phytoplasma 16S rII-C subgroup. So we can classify this strain into phytoplasma 16S rII-C subgroup.
%K Opuntia salmiana porm
%K Witches'-broom
%K Phytoplasma
%K 16S rRNA gene
%K Sequence analysis<br>仙人掌
%K 丛枝
%K 植原体
%K 16SrRNA基因
%K 序列分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=C39E3BB1398C32B7&yid=14E7EF987E4155E6&vid=2001E0D53B7B80EC&iid=B31275AF3241DB2D&sid=99A964928ADB4E67&eid=6CDD207A90CE1EEC&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=9