%0 Journal Article
%T Loop-mediated isothermal amplification assay for rapid detection of Enterobacter sakazakii in powdered infant formula<br>改良环介导等温扩增技术快速检测婴儿配方奶粉中的阪崎肠杆菌
%A Lianxia Hu
%A Wei Zhang
%A Xianzhou Zhang
%A Yaowu Yuan
%A Yunzhe Zhang
%A Huiyan Zhang
%A Xiaoyan Ma
%A Xudong Su
%A <br>胡连霞
%A 张伟
%A 张先舟
%A 袁耀武
%A 张蕴哲
%A 张会彦
%A 马晓燕
%A 苏旭东
%J 微生物学报
%D 2009
%I 
%X Abstract: Objective] A loop-mediated isothermal amplification (LAMP) technology with two loop primers was developed for rapidly detecting Enterobacter sakazakii in powdered infant formula. Methods] Sequences of 16S-23S rRNA of Enterobacter sakazakii (ATCC29544) were used as target sequences, to design outer primers, inner primers and loop primers. We judged the results of detection, through visible to the naked eye of the white precipitate. Results]The sensitivity of the LAMP assay was 0.101 CFU/mL; the detection limit of artificial contamination was 1.1 CFU/g. The detection could be finished about an hour from dealing with the sample to report the results with DNA kit. Compared with LAMP, the sensitivity of the PCR assay was 101 CFU/mL and the detection limit of artificial contamination was 1100 CFU/g in three hours. To apply the same method of extracting DNA, from dealing with the sample to report the results, it took about three hours. Conclusion] Therefore, this LAMP-based assay is sensitive and fast. These results indicate that LAMP can provide a rapid yet simple test for the detection of Enterobacter sakazakii in powdered infant formula.
%K LAMP<br>环介导等温扩增
%K 检测
%K 阪崎肠杆菌
%K 婴儿配方奶粉
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=8AB721389485D033CBD555363F8F38CA&yid=DE12191FBD62783C&vid=2A3781E88AB1776F&iid=38B194292C032A66&sid=73648F51F187AC5E&eid=9C82B18080268586&journal_id=0001-6209&journal_name=微生物学报&referenced_num=4&reference_num=16