%0 Journal Article
%T Isolation and identification of an endophytic fungus of Polygonatum cyrtonema and its antifungal metabolites<br>一株多花黄精内生真菌的鉴别及其抗菌代谢产物
%A Ying Wang
%A Guoping Wang
%A Liwei Wang
%A Xuhui Xu
%A Jing Xi
%A Xuefang Huang
%A Yougui Wu
%A Chulong Zhang
%A <br>汪滢
%A 王国平
%A 王丽薇
%A 徐旭辉
%A 夏静
%A 黄雪芳
%A 吴友贵
%A 章初龙
%J 微生物学报
%D 2010
%I 
%X Abstract: Objective] Endophytic fungi in medicinal plants produce a variety of bioactivity compounds. In this study, an endophytic fungus zjqy610 with antifungal activity was isolated from Polygonatum cyrtonema in Zhejiang Qingyuan Baishanzu Mountain nature reserve. Methods] Strain zjqy610 was identified as Penicillium canescens based on the morphology and its rDNA sequence analysis. Three antifungal compounds were isolated from the fermentation broth of zjqy610 through normal-phase silica gel column chromatography and gel (Sephadex LH-20) column chromatography, traced by ultraviolet light or iodine vapor with bioassay-guided fractionation. Results] These three compounds were elucidated as o-acetylbenzeneamidinocarboxylic acid (zjqy610B-g-3), griseofulvin (zjqy610D-4) and naphtho 1,2-b] furan-3 -carboxylic acid, 4-hydroxy-5-methoxy-2-methyl-(zjqy610F-2) based on mass spectrometry and nuclear magnetic resonance spectroscopy. The antifungal activity assays showed that the three compounds had inhibitory to variety of plant pathogenic fungi. Compound zjqy610D-4 had strong antifungal activity against Botrytis cinerea, Colletotrichum orbiculare, Didymella bryoniae and Sclerotinia sclerotiorum. EC50 was 0.68, 0.38, 0.91 and 0.61 mg/L, respectively. Conclusion] Zjqy610D-4 is deserved to develop an agricultural antibiotics.
%K Keywords: Polygonatum cyrtonema
%K Endophytes
%K Penicillium canescens
%K antifungal activity
%K structure elucidation<br>关键词：多花黄精
%K 植物内生菌
%K 变灰青霉
%K 抗真菌活性
%K 结构鉴定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=AC8528DEEB9DCBE30AA0E2D2641E642A&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=5D311CA918CA9A03&sid=E1D875FA50925809&eid=9E5DBB98644FDC52&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0