%0 Journal Article
%T Study on Rotavirus detection by nucleic acid sequence based amplification<br>轮状病毒NASBA检测研究
%A KOU Xiao-xia
%A WU Qing-ping
%A FAN Hong-ying
%A <br>寇晓霞
%A 吴清平
%A 范宏英
%J 微生物学报
%D 2006
%I 
%X Rotavirus is one of the major cause of the viral gastroenteritis throughout the world. A nucleic acid sequence-based amplification(NASBA) technique for the detection of rotavirus in faecal specimens was developed and compared to the RT-PCR technique. Primers were designed according to the high conserved region of VP7 gene. Amplicons were detected by denaturating agarose gel, and then demonstrated by Northern hybridization with digoxigenin-labeled oligonucleotide probe. The anticipative specific amplification product is 392bp,and no nonspecific products appear even the concentration of nontarget nucleic acid as high as 1 microg/microL. A detection limit of 50 pg target RNA/mL is obtained when the optimal amplification time of 3h used.The NASBA assay will be a favourable alternative to RT-PCR for the investigation of rotavirus outbreaks as a routine diagnostic test in the near future because it is shown to be highly sensitive, specific and do not require specialized equipment.
%K NASBA
%K Rotavirus
%K Detection
%K Gastroenteritis
%K RT-PCR<br>NASBA
%K 轮状病毒
%K 检测
%K 胃肠炎
%K RT-PCR
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=6B353CA580643888&yid=37904DC365DD7266&vid=D997634CFE9B6321&iid=0B39A22176CE99FB&sid=A5B34D9E8FDA439A&eid=6C069F4B248105A5&journal_id=0001-6209&journal_name=微生物学报&referenced_num=2&reference_num=10