%0 Journal Article
%T Application of gyrB in the identification of closely related bacteria-A review<br>促旋酶(gyrase) B亚单位基因gyrB在鉴别细菌近缘种中的应用
%A Xianmei Li
%A Xiaofen Wang
%A Hongyan Yang
%A Xiuzhi Gao
%A Zongjun Cui
%A <br>李献梅
%A 王小芬
%A 杨洪岩
%A 高秀芝
%A 崔宗均
%J 微生物学报
%D 2008
%I 
%X The single-copied gyrB gene, encoding the subunit B of gyrase and distributing universally in all bacteria, has the average substitution rate of 0.7%-0.8% per million years. It's proved that this region could be used to discriminate and identify the closely related species of different bacteria such as Pseudomonoas, Bacillus, Vibrio, Enterobacteriaceae, Mycobacteria, Aeromonas, Lactic acid bacteria et.al. It also can be used of quantitative or restriction fragment analysis of bacteria with the aid of species-specific primers or combined with DGGE (denaturing gradient gel electrophoresis). It really brings new promise for the identifying closest isolates or fingerprinting population due to overcoming the shortage of undistinguishing them acutely with the non-protein-encoding genes such as 16S rDNA or ITS (internal transcribed spacer) DNA. It's an important new molecular marker for researches of closely related species and becoming an attractive topic in current microbial research world.
%K gyrase
%K gyrB
%K closely related species
%K phylogenetics<br>促旋酶
%K gyrB
%K 近缘种
%K 系统发育
%K 促旋酶
%K 亚单位基因
%K 鉴别
%K 细菌
%K 近缘种
%K 应用
%K review
%K related
%K identification
%K 新靶标
%K 系统发育分析
%K 热点
%K 外用
%K 指纹图谱
%K 群体
%K 缺陷
%K 间隔序列
%K rDNA
%K 蛋白编码基因
%K 动态
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=F33B1EB7B5070CF1B8196BC6E8FE3C56&yid=67289AFF6305E306&vid=B6DA1AC076E37400&iid=94C357A881DFC066&sid=80BBC722D530DB8D&eid=C19D5524C51D7FE4&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=36