%0 Journal Article
%T Expression of bursin receptor on membranes of hybridoma cell and Identification of binding peptide of bursin from 12-merrandom phage display peptide library<br>囊素与杂交瘤细胞的结合及结合肽的鉴定
%A Chen Wang
%A Zhanqin Zhao
%A Mingfu Niu
%A Chunjie Zhang
%A Xiangchao Cheng
%A Puyan Chen
%A <br>王臣
%A 赵战勤
%A 牛明福
%A 张春杰
%A 程相朝
%A 陈溥言
%J 微生物学报
%D 2010
%I 
%X Abstract: Objective] Bursin (BS) could greatly promote the production of monoclonal antibody in hybridoma cell. We studied the interaction between BS and hybridoma cell. Methods] Fluorescence microscopy, Laser Scanning Confocal Microscope (LSCM) and fluorescence activated cell sorter (FACS) were used. Results] Fluorescence microscopy revealed specific binding of FITC-BS to hybridoma cell. FACS analysis demonstrated that the binding was specific, saturable and reversibility. BS was used as target protein to screen its binding peptides from 12-mer random phage display peptide library. After four rounds of biopanning, 20 phage clones were randomly selected and identified. ELISA and competitive inhibition test results indicated that 2 phage clones were identified as positive clones. The amino acid sequences analysis shown that the sequences were ACTKHLCLLQPL or MSCNDTLCLLPN, which sharing a conservative sequence LCLL. In vitro experiments suggested that the two binding peptides can inhibit BS specific binding to hybridoma cell. Conclusion] These results confirmed that existence of BS receptor in the membrane of hybridoma cell, which involved in hybridoma cell secreting monoclonal antibody signal pathway.
%K Key words: bursin
%K hybridoma cell
%K receptor
%K binding peptide
%K peptide library<br>关键词：囊素
%K 杂交瘤细胞
%K 受体
%K 结合肽
%K 肽库
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=D841DB1FF245C47739636520484D58D0&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=0B39A22176CE99FB&sid=B7BFA4B351E4C682&eid=CAA7BAE04CB631A1&journal_id=0001-6209&journal_name=微生物学报&referenced_num=1&reference_num=0