%0 Journal Article
%T INDUCTION AND PURIFICATION OF TWO EXTRACELLULAR CHITINASES FROM BEAUVERIA BASS1ANA<br>球孢白僵菌两种胞外几丁质酶的诱导和纯化
%A Peng Renwang Guan Kaomei Huang Xiuli
%A <br>彭仁旺
%A 管考梅
%A 黄秀梨
%J 微生物学报
%D 1996
%I 
%X Chitinases were induced when Beauveria bassiana CH-1316,a UV mutagenic mutant strain, was first grown in a complete medium to pre-logarithmic growth phase and then transferred to a medium containing colloidal chitin as a sole carbon and nitrogen source. Culture filtrates were collected about 20-25h after transferring, and this just occurred at the logarithmic growth phase and the peak time of chitinase activities. Two extracellular chitinases were purified to homogeneous by (NH4)2SO4 precipitation, DEAE-cellulose chromatography and gel filtration. Chitinase 1 showed mixed activities of endo-and exo-chitinase,while chitinase 2 was an endo-chitinase. The molecular weight of two chitinases were determined to be 52 000 and 39 000 by gel filtration.
%K Beauveria bassiana
%K Extracellular chitinases
%K Purification
%K Molecular weight<br>球孢白僵菌
%K 胞外几丁质酶
%K 纯化
%K 分子量测定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=6D70FC6F56196D686F1E037DE906D78F&yid=8A15F8B0AA0E5323&vid=933658645952ED9F&iid=0B39A22176CE99FB&sid=89F76E117E9BDB76&eid=6270DC1B5693DDAF&journal_id=0001-6209&journal_name=微生物学报&referenced_num=17&reference_num=1