%0 Journal Article
%T Study on the screening of lactamase and its fermentation conditions<br>产γ_内酰胺水解酶菌株的筛选及发酵条件研究
%A LI Hai-quan
%A SU Lei
%A YANG Liu
%A WANG Jian-jun
%A ZHENG Guo-jun
%A <br>李海泉
%A 苏磊
%A 杨柳
%A 王建军
%A 郑国钧
%J 微生物学报
%D 2006
%I 
%X (-)-gamma-Lactam is one of the key starting materials for the synthesis (-)-cabovir and (-)-abacavir, which serve as two powerful antiviral agents. The production of gamma-lactam using enzymatic resolution of racemic gamma-lactam was attempted in the paper. 20 out of 69 strains capable of producing lactamase were screened from soil samples collected in several districts throughout Beijing; particularly N-actylphenylalanine was used as sole carbon source in the screening method. It was found by chiral HPLC analysis that strain L29-9 was able to enantioselectively hydrolyze the (+)-isomer in the racemic lactam, thus giving the desired (-)-gamma-lactam with high enantiomeric excess (> 70%). Ferment conditions of strain L29-9, including carbon source, nitrogen source, pH, and culturing time, were studied and the optimum conditions were as follows: citric acid 2 g/L, yeast extract 5 g/L, pH 7.0, culturing for 40h. Biotransformation using whole cells of the strain was inducted at 30 degrees C for 12h, giving (-)-gamma-lactam as enantiopure product with yield up to 40% and 99.5% e.e.
%K 7-1actam
%K 2-azabicyclo[2
%K 2
%K 1 ]-hept-5-en-3-one
%K Resolution<br>γ-内酰胺
%K 2-氮杂双环-[2.2.1]-庚烷-5-烯-3-酮
%K 拆分
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=F29F129A45586303&yid=37904DC365DD7266&vid=D997634CFE9B6321&iid=E158A972A605785F&sid=F16C0F639D87527E&eid=E406B4E9A1BA9D8C&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=8