%0 Journal Article
%T Construction of integrative vector of Candida utilis<br>产朊假丝酵母整合表达载体的构建
%A Honglan Yang
%A Wei Wang
%A Huifang Bao
%A Chunsheng Cui
%A Shuang Hu
%A <br>杨红兰
%A 王炜
%A 包慧芳
%A 崔春生
%A 胡爽
%J 微生物学报
%D 2009
%I 
%X Abstract: Objective] Xylanase is ahemicellulase. It can reduce xylan, which is the abundance resource on our earth. It is important in industries to obtain recombinant strains secreting xylanase to degrade hemicelluloses and produce xylose as desired products. We constructed an integrative vector of Candia utilis for xylanase expression. Methods] On the basis of plasmid pBR322, an integrative expression vector pGLR9K for Candida utilis was constructed that contained strong promoter of GAP, 18S rDNA sequence for homologous recombination and mutated L41 gene as CYH resistant marker. Recombinant expression vector pGLXR was constructed by adding xylanase gene to the pGLR9K and then was transferred into C. utilis. Results] Both intracellular and extracellular xylanase activities were detected in transgenic strains. Conclusion] This system can be used for exogenous genes expression in transgenic C. utilis.
%K 产朊假丝酵母
%K 同源整合
%K 载体构建
%K 整合表达
%K 木聚糖酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=996187B136F192D2FEADAFCAC5A150D3&yid=DE12191FBD62783C&vid=2A3781E88AB1776F&iid=38B194292C032A66&sid=EC34D52BE81085CE&eid=892C6E385D640C1E&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=24