%0 Journal Article
%T Correlation of the neutralization index in chicken embryo with the homolo-gies of F and HN gene of different Newcastle-Disease isolates<br>新城疫不同毒株交叉鸡胚中和指数及其与F和HN基因变异的相关性
%A Zhuoming Qin
%A Huaiying Xu
%A Wenjun Ouyang
%A Youling Wang
%A Lili Wang
%A Xiaoyuan Yuan
%A Leitao Tan
%A <br>秦卓明
%A 徐怀英
%A 欧阳文军
%A 王友令
%A 王莉莉
%A 袁小远
%A 谭雷涛
%J 微生物学报
%D 2008
%I 
%X Thirteen prevailed Newcastle-disease viruses (NDV) isolated in China during 2001-2004 were purified by chick embryo fibroblast (CEF) plaque assay and characterized pathotypically and genotypically. The biological tests showed that these viruses were highly virulent. Sequence analysis based on the variable region (nucleotide 47-420) of the F gene indicated that of the 13 NDV isolates 2 belonged to genotype II, 2 to genotype IX and 9 to genotype VII. Isolates with genotype VII shared 94.6%-99.3% nucleotide (nt) homology with the F gene, whereas for genotype VII and La Sota was only 82.7%-84.1%. In addition, these NDV isolates all shared 95.2%-100% nt homology with the hemagglutinin-neuraminidase (HN) gene, whereas only 79.1%-84.3% compared these viruses with La Sota. The cross neutralization assays were done using positive serums in specific pathogen free (SPF) chicken embryos respectively. Correlation of the neutralization index in chicken embryo with the homologies of F and HN gene of different NDV isolates were analyzed by SPSS8.0 software. The result showed that the neutralization index was closely correlated with nt sequence (P < 0.01, r = 0.35) or deduced amino acid sequence (P < 0.01, r = 0.34) of the HN gene, whereas weekly correlated (P < 0.05, r = 0.20 or 0.19) with the F gene, and non-correlated with 374 nt segment. This implied that the genetic mutations of HN resulted in antigenic variations of these viruses and the search for new vaccines would be necessary.
%K Newcastle disease virus
%K F gene
%K HN gene
%K antigenicity
%K correlation<br>新城疫病毒
%K F基因
%K HN基因
%K 抗原性
%K 相关性
%K 新城疫
%K 不同毒株
%K 鸡胚
%K 指数
%K 基因变异
%K 相关性
%K different
%K gene
%K chicken
%K embryo
%K index
%K neutralization
%K 疫苗株
%K 影响
%K 抗原性
%K 分子变异
%K 核甘酸
%K 结果
%K 相关比较
%K 同源性
%K 氨基酸
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=CEB96C0722336DB2D0407CBF88AE23C4&yid=67289AFF6305E306&vid=B6DA1AC076E37400&iid=0B39A22176CE99FB&sid=6ED15D8DCB279BC4&eid=FD7C952458BFB5D8&journal_id=0001-6209&journal_name=微生物学报&referenced_num=1&reference_num=16