%0 Journal Article
%T Screening cellular proteins interacted with M2 protein of influenza A virus by Coimmunoprecipitation<br>免疫共沉淀筛选细胞内与A型流感病毒M2蛋白相互作用的蛋白
%A Yaodong Li
%A Zhenhong Guan
%A Jinghua Yan
%A <br>李耀东
%A 关振宏
%A 严景华
%J 微生物学报
%D 2009
%I 
%X Abstract: Objective] To screen cellular protein interacted with influenza A M2 protein(A/M2). Methods] we cloned A/M2 gene fragment into pCAGGS-CFlag vector, and the resulting plasmid was transfected into human embryonic kidney (HEK) 293T cells.The recombinant Flag fusion protein,A/M2-Flag was absorbed specificly by Anti-Flag Monoclonal Antibody M2-Conjugated Agarose beads, we loaded the beads on 12% SDS-PAGE after we washed it with lysis buffer. Silver staining of the gel revealed that several proteins were co-purified with A/M2.To identify the proteins,we excised the protein bands and analysed them by mass spectroscopic sequencing. Results] We got two kinds of proteins, ataxin 10 and eukaryotic initiation factors(eIFs). Conclusion] Interaction between Ataxin 10 and A/M2 would explain why inflenza virus infection or influenza vaccine innoculation causes acute cerebellar ataxia. A/M2 interacting with eIFs would imply that A/M2 is involved in the regulation of influenza virus protein synthesis.
%K Keyword: coimmunoprecipitation
%K A/M2
%K mass spectrometry
%K protein interaction<br>关键词：免疫共沉淀
%K A型流感病毒M2蛋白
%K 质谱
%K 蛋白相互作用
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=C39826A78DDBCC8F6621D77FC6BFFA5B&yid=DE12191FBD62783C&vid=2A3781E88AB1776F&iid=5D311CA918CA9A03&sid=5A9F0976AE79CB6F&eid=126825C4749D880D&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=13