%0 Journal Article
%T Sited-directed mutagenesis of hCu ,Zn-SOD gene and its expression in Synechococcus sp. PCC7942<br>人铜锌超氧化物歧化酶基因改良及在聚球藻中表达
%A ZHOU Zan-hu
%A ZHANG Hai-yan
%A LIU Ren-hai
%A ZHANG Jun
%A ZHOU Ke-fu
%A XU Hong
%A <br>周赞虎
%A 张海艳
%A 刘仁海
%A 章军
%A 周可夫
%J 微生物学报
%D 2006
%I 
%X The Cys111 genetic code of human copper/zinc superoxide dismutase (hCu, Zn-SOD) gene in the pESOD plamid was mutated into the Ala111 code with site-directed mutagenesis, and then the plamid pESODT111 which contained groESL promoter, mutated hCu, Zn-SOD gene, rbcS-polyA terminator and reporter gene (Kanr) was constructed and transduced into Synechococcus sp. PCC7942 with homologous recombination platform. The results of PCR and DNA sequence analysis showed that the target nucleotide had been genetically integrated into genome DNA of the host cell. SDS-PAGE, Western blot and Pyrogallol autoxidation assay confirmed that the transformant strains expressed the mutated hCu, Zn-SOD protein. And the level of the mutated hCu, Zn-SOD protein reached a value of 3.61% of the total soluble protein. Furthermore, the transformants still retained 95% activities of SOD after 30 minutes at 80 degrees C environment, it indicated that the mutated hCu, Zn-SOD protein could endure higher temperature than the natural one.
%K hCu
%K Zn-SOD
%K Sited-directed mutagenesis
%K Synechococcus sp
%K PCC7942
%K Expression
%K Hot stability<br>hCu
%K Zn-SOD
%K 定点突变
%K 聚球藻
%K 表达
%K 热稳定性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=57ED0EB680404A5D&yid=37904DC365DD7266&vid=D997634CFE9B6321&iid=CA4FD0336C81A37A&sid=2922B27A3177030F&eid=EB552E4CFC85690B&journal_id=0001-6209&journal_name=微生物学报&referenced_num=3&reference_num=12