%0 Journal Article
%T Bacterial Diversity in Zabuye Salt Lake Tibet by Culture-Independent Approaches<br>西藏扎布耶盐湖细菌多样性的免培养技术分析
%A Xianhui Zhang
%A Fanjing Kong
%A <br>张现辉
%A 孔凡晶
%J 微生物学报
%D 2010
%I 
%X Objective]To get knowledge about bacterial diversity in Zabuye salt lake with the culture-independent approaches. Methods]Total DNA was extracted from sediments sample of Zabuye salt lake, Tibet. The 16S rRNA gene was amplified by PCR with bacterial primer f530/r1492. Then the plasmid library of 16S rRNA genes was constructed. The positive clones were screened on plates with IPTG/X-gal/Ap. Amplified ribosomal DNA restriction analysis ( ARDRA) was carried out with restriction enzymes Hae III, Hha I. With t...
%K Keywords: Zabuye Salt lake
%K bacterial diversity
%K Culture-independent approaches
%K 16S rRNA gene
%K ARDRA<br>关键词：扎布耶盐湖，细菌多样性，免培养技术
%K 16S
%K rRNA基因
%K ARDRA
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=ABBC9A9DD8E6690B458873A5499FD03B&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=38B194292C032A66&sid=9DC563A0FEFC04F9&eid=4B168891B5E5FB30&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0