%0 Journal Article
%T Exploring the Antifungal Activity of Bacillus amyloliquefaciens NK10.BAhjaWT
解淀粉芽孢杆菌NK10.BAhjaWT抑真菌作用的研究
%A HAO Jian-An
%A CAO Zhi-Hui
%A ZHAO Feng-Mei
%A GAO Wei-Hu
%A XU Hai-Jin
%A BAI Yan-Ling
%A ZHANG Xiu-Ming
%A QIAO Ming-Qiang
%A
郝建安
%A 曹志辉
%A 赵凤梅
%A 高卫华
%A 徐海津
%A 白艳玲
%A 张秀明
%A 乔明强
%J 微生物学报
%D 2008
%I
%X Bacillus are well known antibiotic producers. In this study, dozens of Bacillus strains from different sources were screened. Among them, a strain with strong antifungal activity was found. With 16S rDNA test and Biolog assay, this strain was identified to be Bacillus amyloliquefaciens. The fermentation conditions were optimized in small conical flasks. After ammonium sulfate salting out, dialysis, freezing vacuum dehydration, the crude protein extracts were obtained. The thermal stability, pH stability, protease stability, ion stability and antifungal spectrum of this protein were studied further. Scanning electronic microscope was also used to explore the antifungal mechanism.
%K Bacillus amyloliquefaciens
%K Antifungal protein
%K Stability
%K Antifungal spectrum
解淀粉芽孢杆菌
%K 抑真菌蛋白
%K 稳定性
%K 抑真菌谱
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=974A29B04C01F78E1A7922E126649AD7&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=B31275AF3241DB2D&sid=A1DCE313EDFB3D3A&eid=802F367A9CC18C1A&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0