%0 Journal Article
%T Effects of 2-phenylethanol on physiological and biochemical characteristics of Saccharomyces cerevisiae<br>2-苯乙醇对酿酒酵母生理生化特性影响
%A Wang Hang
%A Meng Chun
%A Shi Xian-Ai
%A Guo Yang-Hao
%A <br>王航
%A 孟春
%A 石贤爱
%A 郭养浩
%J 微生物学通报
%D 2012
%I 
%X Objective] To provide useful basis for enhancing the biosynthesis of 2-phenylethanol (PEA), we studied the variation of physiological and biochemical characteristics of Saccharomyces cerevisiae sp. strain R-UV3 treated on varied concentrations of PEA. Methods] Morphological observation was performed by a transmission electron microscope. Membrane permeabilization, intracellular reactive oxygen species (ROS) and mitochondrial membrane potential after staining with propidium iodide, dihydroethidium and rhodamine 123 respectively were investigated at the single-cell level with flow cytometry. The expression of aro10 gene was determined by real-time fluorescence quantitative PCR. Results] With the increasing PEA concentration (0?4.0 g/L), the membrane permeabilization, glycometabolism and expression of aro10 gene in the yeast cell decreased and mitochondrial membrane potential increased. ROS increased when PEA concentration was below 3.0 g/L and decreased when PEA concentration was beyond 3.0 g/L. The physiological and biochemical characteristics of the yeast, such as membrane permeabilization, glycometabolism and expression of aro10 gene, varied notably as PEA concentration increased from 2.4 g/L to 3.0 g/L. Conclusions] The aqueous PEA concentration should be controlled at 2.4?3.0 g/L when in situ PEA removal technology was performed.
%K 2-phenylethanol
%K Saccharomyces cerevisiae
%K Physiological and biochemical characteristic
%K Flow cytometry<br>2-苯乙醇
%K 酿酒酵母
%K 生理生化特性
%K 流式细胞术
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=829D0ABBF0B6FE5A6027C9BD183A71B0&yid=99E9153A83D4CB11&vid=7C3A4C1EE6A45749&iid=B31275AF3241DB2D&sid=B9F7F8AE8D5435F7&eid=0FFF013C9C1B022F&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=10