%0 Journal Article
%T Screening of harmine tolerance/degrading bacteria from camel rumen<br>骆驼瘤胃耐受/降解去氢骆驼蓬碱细菌的筛选
%A Dengdi An
%A Yanlei Zhu
%A Jing Tang
%A Yongxia Ye
%A Xianchun Zeng
%A <br>安登第
%A 朱艳蕾
%A 唐静
%A 叶永霞
%A 曾献春
%J 微生物学报
%D 2010
%I 
%X Abstract: Objective] Peganum harmala, a famous traditional Chinese drug, contains a variety of alkaloids and toxic for many animals. Camels mainly live in desert or semi-desert areas, with the robust gastro- intestine system in digesting various feed including toxic plants without disease symptoms. Methods] Camel rumen content was used as the inoculant to inoculate medium M98-5 which contains 100 mg?L-1 harmin and cultivated for 5 days. Upto 5 subculturings, strains that could degrading or tolerant harmine were isolated. Their conversion activity was determined by thin-layer chromatography. The taxonomic position of the strains were identified based on 16S rRNA sequences analysis. Results] 15 out of the 29 isolates have harmine degrading activity. Most of the isolates are identified as the members of the Genera Lactobacillus (16 strains, 55%), Shigella (7 strains, 24%) and Bacillus (4 strains, 13.8%). Only one strain belong to genus Enterococcus and one belong to genus Megasphaera. Conclusion] The results indicated that the harmine tolerance/degrading communities of camel rumen are limited and only Lactobacillus have harmine-degrading activity.
%K Keywords: Rumen microbe
%K Plant toxins
%K tolerance/degrading
%K Camels<br>关键词：瘤胃微生物
%K 植物毒素
%K 耐受/降解
%K 骆驼
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=766017BB3618BFD8A89055F7A267E7F1&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=5D311CA918CA9A03&sid=FC20C40D27903A85&eid=66156A49F03BF135&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0