%0 Journal Article
%T Construction and characterization of recombinant Salmonella enterica serovar choleraesuis vaccine strain expressing heterologous antigen of Pasteurella multocida toxin<br>表达T~+Pm保护性抗原的重组猪霍乱沙门氏菌C500株的构建及其生物学特性
%A Zhanqin Zhao
%A Chen Wang
%A Ke Ding
%A Xiaofeng Li
%A Bin Wu
%A Shun Lu
%A Chunjie Zhang
%A Xiangchao Cheng
%A <br>赵战勤
%A 王臣
%A 丁轲
%A 李晓峰
%A 吴斌
%A 卢顺
%A 张春杰
%A 程相朝
%J 微生物学报
%D 2010
%I 
%X Abstract: Objective] This study was aimed to construct and characterize Salmonella vaccine strain C500 expressing the recombinant Pasteurella multocida toxin (PMT) antigen by the Asd+ balanced-lethal host-vector system. Methods and results] The DNA fragment encoding C terminal of PMT was cloned downstream from the beta-lactamase signal sequence in the multicopy Asd+ pYA3493 vector to create pYA-PmtC. Fermentation patterns, serotype, and mean generation time of the vaccine strain C500 harboring pYA-PmtC (named with C501(pYA-PmtC)) were identical to those of the parent strain C500. The recombinant pYA-PmtC plasmid was very stable in C501(pYA-F1P2), which expressed secretorily a large amount of the recombinant PMT antigen (named with rPmtC). The virulence of C501(pYA-PmtC) with LD50 of 8.5×106 CFU was a little lower than C500 with LD50 of 4.4×106 CFU based on the method of Reed and Muench. All piglets inoculated with C501(pYA-PmtC) or C500 survived, and had no signs of disease during the entire experimental period. No significant differences were found between these two groups. Conclusion] The recombinant vaccine strain C501(pYA-PmtC) had a series of biological characteristics silimar to the parent vaccine strain C500. It is likely that C501(pYA-PmtC) could be adapted to develop multivalent recombinant Salmonella vaccine against both infections with S. enterica serovar Choleraesuis and toxigenic P. multocida.
%K Keywords: Salmonella enterica Serovar Choleraesuis C500 strain
%K toxigenic Pasteurella multocida
%K Asd+ balanced-lethal host-vector system
%K recombinant vaccine<br>关键词：猪霍乱沙门氏菌C500株，产毒巴氏杆菌，Asd+平衡致死系统，重组疫苗
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=09AEEC87575D8AB465FB1E3851E133E1&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=CA4FD0336C81A37A&sid=C753EB8AC8F551B9&eid=C3BF5C58156BEDF0&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0