%0 Journal Article
%T Preparation of the Vaccine with Inactivated Feline Panleukopenia Virus Isolated from Tiger and the Preliminary Application<br>虎源猫泛白细胞减少症病毒灭活疫苗的制备及初步应用
%A Yali Yu
%A Ming Zhou
%A Jin Zhang
%A Yuping Hu
%A Ligang Wang
%A Yinan Liu
%A Dan Liu
%A Xianzhu Xia
%A <br>于亚丽
%A 周明
%A 张进
%A 华育平
%A 王立刚
%A 刘熠楠
%A 刘丹
%A 夏咸柱
%J 微生物学报
%D 2009
%I 
%X Abstract: Objective] To prepare a vaccine with inactivated Feline Panleukopenia Virus (FPV) isolated from Siberian tigers and to evaluate its immunological effect. Methods] FPV-HLJ, an FPV strain previously isolated from Siberian tiger in our lab was used to inoculate cat kidney cell line F81 with dose 1/10(v/v) using the synchronizing inoculation method. Inoculated F81 cell line was cultured at 37°C and collected when cytopathic effect was up to 75%. Viral suspension was inactivated by using formaldehyde for 24 h and inactive vaccine preapred by adding aluminium hydroxide gel as adjuvant to the suspension. The inactive vaccine was applied to 2- month old kitten tigers of hypodermically after protection effect was proved in 2-month old nonimmunized cats by using the same vaccination procedure. Results] Antibody level in vaccinated cats revealed an increasing trend that the valence of antibody reached 1:1024~2048 after three times of vaccination. All vaccinated cats survived challenges of virulent FPV virus. The valence of antibody reached 1:1024 in most vaccinated tigers 15 days after the third vaccination. Conclusion] The results indicated that the inactivated vaccine can produce immunoprotection for tigers.
%K Keywords: tiger
%K Feline Panleukopenia Virus
%K inactivated vaccine
%K antibody detection<br>关键词：虎
%K 猫泛白细胞减少症病毒
%K 灭活疫苗
%K 抗体检测
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=18F642B5D62AEBDB9DD3B89DBB45815A&yid=DE12191FBD62783C&vid=2A3781E88AB1776F&iid=94C357A881DFC066&sid=5DD21DF25EF52D4A&eid=AEE2F90BC0DB5F35&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=16