%0 Journal Article
%T Improved culturability of soil bacteria using proper combination with various culturing medium<br>不同培养基组合提高土壤细菌可培养性的研究
%A HU Yuan-sen
%A LI Cui-xiang
%A SUN Fu-lin
%A WU Kun
%A JIA Xin-cheng
%A <br>胡元森
%A 李翠香
%A 孙富林
%A 吴坤
%A 贾新成
%J 微生物学报
%D 2007
%I 
%X To isolate more unique and previously unrecognized bacteria in soil samples, the culture difference under three incubation modes was investigated by using trophic, low-nutrient broth and soil extract as growth medium. Plate count proved that the oligotrophic medium resulted in a slow growth and consecutive colony formation over the course of incubation. On the 5th day, the most number of colony-forming unit was found on trophic LB and low-nutrient R2A, which was approximate 5 times as many as that isolated on 0.1×LB. Of the 7 media, LB broth harvested the maximum bacterial communities, and novel species could be isolated as the nutrient was diluted to appropriate extent. The DGGE patterns of oligotrophic and rich nutrient culture collection displayed low similarity, however, the bands at various lanes exhibited complementary effect. When cultivated with static flask, LB and R2A media obtained more bacterial species, which concluded most species isolated by the other five media. Under the test tube incubation mode, the most species was also found in LB medium except some appeared only on R2A and TSB. Apparent bacterial communities difference could be detected between R2A, LB and TSB media. The experiment data may contribute much to the special medium design as well as improvement of bacterial culturability by using proper medium.
%K bacterium
%K culturability
%K medium
%K DGGE<br>细菌
%K 可培养性
%K 培养基
%K 变性梯度凝胶电泳
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=D3B9F308AD66EEA0&yid=A732AF04DDA03BB3&vid=F4B561950EE1D31A&iid=94C357A881DFC066&sid=8047434EAE0B2346&eid=3E851F4852A9516C&journal_id=0001-6209&journal_name=微生物学报&referenced_num=2&reference_num=17