%0 Journal Article
%T Construction of an engineered Acidithiobacillus caldus with high-efficiency arsenic resistance<br>一株高效抗砷喜温硫杆菌工程菌的构建
%A ZHAO Qing LIU Xiang-mei~ ZHAN Yang LIN Jian-qun YAN Wang-ming
%A <br>赵清
%A 刘相梅
%A 詹杨
%A 林建群
%A 颜望明
%J 微生物学报
%D 2005
%I 
%X Using the recombinant technique in vitro, a new arsenic resistance plasmid pSDRA4 was constructed by subcloning the arsenic resistance genes from plasmid pUM3 into the wide-host-range IncQ plasmid pMMB24 with the hybrid trp-lac ( tac ) promoter, and followed by deleting the regulative gene of the promoter, the lacIQ gene. Then plasmid pSDRA4 was introduced from E. coli into extremely acidophilic obligately chemolithotrophic Acidithiobacillus caldus by conjugative transfer with a frequency of( 1.444 +/- 0.797) x 10(-4), and the engineered strain of Acidithiobacillus caldus (pSDRA4) for biomining was constructed. The successful transfer demonstrates the development of a conjugational system between strains of E. coli and A. caldus. The recombinant plasmid pSDRA4 is stable in A. caldus. Compared with wild type A. caldus, the level of the arsenic resistance of A. caldus (pSDRA4) is greatly raised from 10mmol/L to 45mmol/L.
%K rsenic resistance gene
%K Acidithiobacillus caldus
%K Conjugative transfer
%K Extremely acidophilic chemolithotrophic bacteria<br>抗砷基因，喜温硫杆菌，接合转移，极端嗜酸性自养细菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=365C7665D3C67A18&yid=2DD7160C83D0ACED&vid=94E7F66E6C42FA23&iid=94C357A881DFC066&sid=DEE640F0CDC9D495&eid=DC06EBDBAF4E06D3&journal_id=0001-6209&journal_name=微生物学报&referenced_num=5&reference_num=15