%0 Journal Article
%T Diversity of actinomycetes in Wuling Mountain<br>武陵山放线菌多样性
%A Yanru Cao
%A Yi Jiang
%A Yiguang Chen
%A Shunkun Tang
%A Sheng Qin
%A Guozhen Zhao
%A Lihua Xu
%A <br>曹艳茹
%A 姜怡
%A 陈义光
%A 唐蜀昆
%A 秦盛
%A 赵国振
%A 徐丽华
%J 微生物学报
%D 2008
%I 
%X OBJECTIVE: To isolate new actinomycetes for discovering compounds of pharmaceutical importance. METHODS: We collected 280 soil samples from virgin forest in Wuling Mountain. In total 1134 actinomycetes were isolated by culture-dependent method. Of them 30 strains were selected and then characterized by phylogenetic analysis based on sequences of 16S rRNA gene. Antimicrobial activities were determined by agar well diffusion method, and genes of type I and II polyketide synthases (PKS I, PKS II), nonribosomal peptide synthase (NRPS) and polyene cytochrome P450 hydroxylase (CYP) were screened by PCR. RESULTS: Thirty strains belonged to 8 families, 13 genera: Streptomyces (>70%), Micromonospora, Dactylosporangium, Catellatospora, Sphaerisporangium, Streptosporangium, Actinomadura, Nonomuraea, Nocardia, Rhodococcus, Arthrobacter, Microbacterium, and Pseudonocardia. There are 5 novel species candidates. Thirty strains showed different antimicrobial activities against one to three bacteria and pathogenic fungi. CONCLUSION: Abundant diversity of actinomycetes existed in the virgin forest of Wuling Mountain which has many new taxa, and Streptomyces is the predominant population. The strains showed high antimicrobial activities and can be further researched for exploiting new compounds of pharmaceutical.
%K actinomycete
%K diversity
%K synthesis genes of secondary metabolites<br>放线菌
%K 多样性
%K 化合物合成基因
%K 武陵山
%K 放线菌
%K Wuling
%K Mountain
%K actinomycetes
%K 开发利用
%K 药物
%K 类群
%K 存在
%K 森林土壤
%K 合成基因
%K 多烯类化合物
%K 抗菌活性
%K 程度
%K 真菌
%K 实验菌
%K 选取
%K 小单孢菌
%K 链霉菌
%K 分离
%K 结果
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=ED1A92556E1BA66E746A2B028431AA24&yid=67289AFF6305E306&vid=B6DA1AC076E37400&iid=DF92D298D3FF1E6E&sid=793F041A4288469A&eid=808D6B9EB5A8B4B4&journal_id=0001-6209&journal_name=微生物学报&referenced_num=1&reference_num=21