%0 Journal Article
%T Detecting hematolysis of Enterococcus from sheep<br>羊源肠球菌溶血性的检测
%A Lichao Kang
%A Sujuan Han
%A Jingmei Wang
%A Xinwen Bo
%A Xun Ma
%A <br>康立超
%A 韩素娟
%A 王静梅
%A 薄新文
%A 马勋
%J 微生物学报
%D 2008
%I 
%X Objective] To characterize hematolysis of Enterococcus from sheep. Methods] Using plate assay (PA), contact hemolysis (CH), supernatant assay (SA), culture hemolysis (CLH) and PCR, we studied hemolysis characteristics of 11 Enterococcus clinical isolates, 30 isolates from healthy sheep, a standard G-Streptococcus and a standard Enterococcus. Results] Rabbit blood and sheep blood were not hemolysising in the 11 clinical isolates analyzed by SA and CH. Of the clinical isolates 63.6% had b-hemolysis with rabbit blood and 36.4% had a-hemolysis with sheep blood analyzed by PA and CLH assay. Of the cylA gene 63.6% was detected in clinical isolates, the sequence of cylA gene was 99.3% homolo-gous with cylA of plasmid pAD1(GenBank accession number: L37110). b-hemolysis had 53.3% in rabbit blood, a-hemolysis and b-hemolysis in sheep blood had 53.3% and 43.3% respectively in 30 healthy sheep initial isolates with PA. Only 6% had hemolytic capacity in rabbit blood after second generations. The cylA gene was not detected in 30 healthy sheep isolates by PCR. Standard Enterococcus strain of a-hemolysis of sheep blood had no hemolysis of rabbit blood. Conclusion] The red blood cells could induce enterococci hemolysis secreting in the bacteria growth. The result was different with the Phenotype and the Genotype.
%K Enterococcus
%K hematolysis
%K detection<br>肠球菌
%K 溶血性
%K 检测
%K 肠球菌
%K 溶血性
%K 同时检测
%K sheep
%K Enterococcus
%K 完全
%K 基因型
%K 生长繁殖
%K 细菌
%K 细胞诱导
%K 情况
%K 检测方法
%K 标准
%K 溶血能力
%K 分离培养
%K 测定
%K 同源性
%K GenBank
%K 序列
%K 扩增产物
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=FB42DF1AE7AC4BB31CB540C6353A6ABF&yid=67289AFF6305E306&vid=B6DA1AC076E37400&iid=DF92D298D3FF1E6E&sid=9C6D270E246D943F&eid=635968EA203846B7&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=19