%0 Journal Article
%T Sequence and evolutionary analysis of VP1 gene of Ovine rotavirus NT<br>羊轮状病毒NT株VP1基因的测序和分子进化分析
%A Yanjun Chen
%A Weiwen Zhu
%A Shuo Sui
%A Xiaowei Zhang
%A Songnian Hu
%A <br>陈艳君
%A 朱伟文
%A 隋硕
%A 张晓伟
%A 胡松年
%J 微生物学报
%D 2009
%I 
%X Abstract: Objective] The ovine rotavirus strain NT isolated from diarrhea lamb in China was considered as a promising vaccine strain. Based on the VP1 gene was one of the important structural proteins of rotavirus, we studied on the evolutional characteristics of VP1. Methods] According to the published conservative sequences of VP1 genes, we designed a pair of specific primers for cloning and sequencing of VP1 gene. Results] Sequencing result showed that the VP1 gene was 3,302 bp in length and the deduced protein was 1,088 aa. Comparison of amino acid sequences revealed that the ORV-NT shared 77.3% - 98.4% similarities with other group A rotaviruses. Furthermore, sequence alignment analysis manifested that amino acid variations mainly occurred in the non-functional regions of VP1 protein. Phylogenetic analysis of VP1 protein showed that the OVR-NT was grouped in the bovine rotavirus clusters, indicating a closer relationship between them. Evolutionary distance of nucleotide sequence and amino acid sequence among VP1 genes of different rotaviruses were calculated, respectively. Analysis of synonymous mutation rate and Non-synonymous mutation rate demonstrated that synonymous substitution was the major pattern of variation in the process of evolution. Conclusion] This was the first report on sequencing and evolutionary distance analysis of VP1 gene of ORV-NT.
%K Keywords: Ovine rotavirus NT strain
%K VP1 gene
%K Sequence alignment
%K Evolutionary distance<br>关键词：羊轮状病毒NT株
%K VP1基因
%K 序列联配
%K 进化距离
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=C3BDCD6D9AFAFEB7BCE9E803D602A658&yid=DE12191FBD62783C&vid=2A3781E88AB1776F&iid=5D311CA918CA9A03&sid=309A221A57FE8496&eid=D7C79E0E439D0B57&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=16