%0 Journal Article
%T NUMERICAL TAXONOMY OF BIFIDOBACTERIA AND HOMOLOGY ANALYSIS OF SOME REPRESENTATIVE STRAINS<br>双歧杆菌的数值分类及代表性菌株同源性的分析
%A Ling Daiwen
%A Zhou Yan
%A Yuan Qiu
%A <br>凌代文
%A 周岩
%A 袁秋
%J 微生物学报
%D 1998
%I 
%X Fifty five sthans of bifldobacteria belonging to Bytdobacterium from various soarees were studied by using ntnnerical taxonoAnc techniques. These organisms included 13 reference sthens and 42 newly isolated sthans (19 sthens of hUInan sources, 25 sthans of edmal somees and 11 strains obtained frOm sewage). Several stalns isolated from the edmals weren't isolated before. overall sindlallhes of the sthens for 75 characters contained morphological, Physiological and biocheAncal charactershcs and anhbacterial suscePtibility were detendned. Dendrognim of numerical analysis based on unweightod Pair cluster average linkage were achieved. Flve clusters were defined at 70% Sm level. The relahonships of these sthens among five clusters were analysed. The stalns of human sourees were basically separeted from the animal sthens, and the sewage strains were distributed betWeen the animal clusters and the human clusters. on the basis of the numerical analysis, the G C content of DNA of som representahve strains among these cltisters were measured. Biohn-labeled DNA segments, comPlementary to variable regions of 16S rRNA, were amplified using PCR as probes. In the dot-blot hybridizahon the relahonships of DNA segmentS among some strains were discussed. The test resultS showed that the homologies among the sthens in same species and various species were differenL The studies are valuable for furtrher researeh in the relahonships of some species axnong bifidobacteria of various sourees.
%K Bifidobacteria
%K Numerical taxonomy
%K Homology<br>双歧杆菌
%K 数值分类
%K DNA同源性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=B1CA673B9B2517D526376C1DB41A73AD&yid=8CAA3A429E3EA654&vid=16D8618C6164A3ED&iid=94C357A881DFC066&sid=4B168891B5E5FB30&eid=15251AE9C02726D3&journal_id=0001-6209&journal_name=微生物学报&referenced_num=2&reference_num=3