%0 Journal Article
%T Detecting pathogenic Aeromonas hydrophila in fish by triplex PCR<br>三重PCR检测鱼类致病性嗜水气单胞菌
%A Yuanwei Wang
%A Cheng Tang
%A Xuehui Yu
%A Ying Wang
%A Hua Yue
%A <br>王远微
%A 汤承
%A 于学辉
%A 王英
%A 岳华
%J 微生物学报
%D 2008
%I 
%X Objective] To develop a rapid PCR method to detect pathogenic Aeromonas hydrophila in fish. Methods] For multiple PCR, three pairs of primers were designed based on the conservative sequences of 16SrRNA genes, aerolysin (aer) gens and serine-protease (ahp) genes of Aeromonas hydrophila. By optimization of PCR conditions and estimation of specificity, sensitivity, detection rate, a triplex PCR assay was established. Results] The assay had a high specificity detecting only pathogenic strains of Aeromonas hydrophila but not other irrelative bacteria. The assay had a high sensitivity with the detection limit as low as 100fg, the detection rate of suspicious clinical samples by this assay was 81.8%, which was noticeably higher than that by bacterial isolation method (40.9%). The detection rate of mimic challenge samples by this assay was 87.5%, which was also noticeably higher than that by bacterial isolation method (67.5%). Conclusion] The simultaneous detection of 16SrRNA gene and two virulent genes in one PCR assay could avoid missed detection possibly caused by PCR with single virulent gene, and provided a useful tool for rapid diagnosis, large-scale quarantine, and epidemiological investigation of the pathogenic Aeromonas hydrophila.
%K Aeromonas hydrophila
%K aer
%K ahp
%K 16S rRNA
%K triplex PCR<br>嗜水气单胞菌
%K aer
%K ahp
%K 16s
%K rRNA
%K 三重PCR
%K 三重
%K 检测方法
%K 鱼类
%K 致病性
%K 嗜水气单胞菌
%K fish
%K 调查
%K 流行病学
%K 检疫
%K 大规模
%K 诊断
%K 误检
%K 漏检
%K 存在
%K 毒力基因
%K 反应管
%K 鲫鱼
%K 细菌分离
%K 黄鳝
%K 疑似
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=776DC9ACA3C5DE4B75BB1EF2F2009B8B&yid=67289AFF6305E306&vid=B6DA1AC076E37400&iid=DF92D298D3FF1E6E&sid=3005465426CC5B70&eid=9822743C2D2BE348&journal_id=0001-6209&journal_name=微生物学报&referenced_num=2&reference_num=18