%0 Journal Article
%T PREPARATION OF MONOCLONAL ANTIBODIES TO BARLEY YELLOW MOSAIC VIRUS<br>大麦黄花叶病毒单克隆抗体的制备
%A Zhao Xiaoli Ma Hong We Xinghua Xu Abing Huang Chunnong
%A <br>赵小立
%A 马红
%A 翁醒华
%A 徐阿炳
%A 黄纯农
%J 微生物学报
%D 1997
%I 
%X Using hybridoma techniques, six hybridoma celllines: 4D6, 4D4, 4F10, 3H6, 3H8 and 4E9 secreting monoclonal antibodies to Shanghai Isolate of Barley yellow mosaic virus (BaYMV) were prepared by fusion between myeloma cells NS-1 and spleen cells of BALB/C mouse immunized with BaYMV. The subclasses of monoclonal antibodies obtained belong to IgG2a. The cross-reaction test with different viruses (SMV, TMV and three isolates of TuMV) showed that six monoclonal antibodies were specific to BaYMV. The titers of six monoclonal antibodies to BaYMV in their cell cultured supernatauts were 1:1-5 x 10(4), and that of 4F10 in ascitic fluid was 1:6.4 x 10(5) by using indirected ELISA test respectively.
%K BaYMV
%K McAb<br>BaYMV
%K McAb
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=72CD0394B7CA24DEABDCD46EFF5F752A&yid=5370399DC954B911&vid=42425781F0B1C26E&iid=94C357A881DFC066&sid=238BD7580EFCC5AE&eid=B79ACB6EBBFC9730&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=6