%0 Journal Article %T Molecular identification of Bacillus strains isolated from extreme dry-sand environment in Qinghai Chaidamu region and its lipopeptide compound analysis
青海柴达木极端干旱沙地分离芽孢杆菌的分子鉴定及拮抗活性分析 %A XIE Yong-Li %A MA Li-Zhen %A XU Zhi-Wei %A DU Zhuo %A GAO Xue-Wen %A
谢永丽 %A 马莉贞 %A 徐志伟 %A 杜卓 %A 高学文 %J 微生物学通报 %D 2012 %I %X Objective] Microbe resource research in extreme environment. Methods] Eight Bacillus strains isolated from Chaidamu extreme dry-sand region in Qinghai Province, were identified by polyphasic molecular taxonomy methods including rep-PCR (BOX-PCR and ERIC-PCR) fingerprints, as well as gyrB and 16S rDNA partial sequence analysis. Antagonistic activity detection and dual culture method screening were used to check antagonistic activity and bio-control efficacy of isolates. MALDI-TOF-MS were used to analyze lipopeptide compound of strains. Results] Eight isolates were identified as Bacillus amyloliquefaciens (6 isolates), Bacillus axarquiensis (1 isolate), Bacillus atrophaeus (1 isolate). All of the isolates presented distinct antagonistic activity and bio-control efficacy to Sclerotinia sclerotiorum. MALDI-TOF-MS results showed that the strain DGL1 (B. amyloliquefaciens) produced Fengycin, strain DGL6 (B. axarquiensis)produced Surfactin, BacillomycinsD and Fengycin, while strain DCD1 (B. atrophaeus) produced Surfactin and Fengycin. Conclusion] The research provided Bacillus resources for further developing and application of bio-control strains adjusting in Qing-Tibet dry-sand extreme environment. %K Qinghai Chaidamu %K Dry-sand extreme environment %K Isolation of Bacillus %K Molecular identification %K Antagonistic activity analysis
青海柴达木 %K 极端干旱沙地 %K 芽孢杆菌 %K 分子鉴定 %K 拮抗活性分析 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=B5A93E035C82D3EF46F60F76E462561E&yid=99E9153A83D4CB11&vid=7C3A4C1EE6A45749&iid=5D311CA918CA9A03&sid=E062BF8B95A95FD2&eid=3395C64999261B75&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0