%0 Journal Article
%T Cloning and expression of egI gene from Penicillium decumbens L-06<br>斜卧青霉L-06内切葡聚糖酶Ⅰ基因的克隆与表达
%A LIU Yun-Tao
%A HAN Xue-Feng
%A LUO Ze-Yu
%A QIU Yu-Feng
%A LONG Min-Nan
%A HU Zhong
%A <br>刘韫滔
%A 韩学凤
%A 罗泽宇
%A 邱玉峰
%A 龙敏南
%A 胡忠
%J 微生物学通报
%D 2012
%I 
%X Objective] The research focus on cloning endoglucanase I (egI) gene from Penicillium decumbens L-06 and expressing in Escherichia coli with high efficiency. Methods] egI gene was cloned from Penicillium decumbens L-06 by RT-PCR method. Recombinant plasmid pET32a-egI was constructed and was transformed into Escherichia coli rosetta(DE3). Recombinant protein with His-tag was expressed in E. coli rosetta(DE3) after induction with IPTG and then was purified with the Ni-NTA affinity chromatography. Results] As expected, the relative molecular mass was approximately 80kD after analyzed by SDS-PAGE and Western blotting. Hydrolysis activity of recombinant protein was assayed by cellulase activity staining and DNS method (2.56 IU/mL). Conclusion] The results achieve the purpose as constructing prokaryotic expression system and expressing egI gene.
%K Penicillium decumbens L-06
%K endoglucanase I
%K Cloning
%K prokaryotic expression<br>斜卧青霉L-06
%K 内切葡聚糖酶Ⅰ
%K 克隆
%K 原核表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=C8C3724E4E2B01E64F3FFB03EF6DE029&yid=99E9153A83D4CB11&vid=7C3A4C1EE6A45749&iid=94C357A881DFC066&sid=629F373584A92903&eid=0462D22862D5DA6F&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0