%0 Journal Article %T Cloning and expression of egI gene from Penicillium decumbens L-06
斜卧青霉L-06内切葡聚糖酶Ⅰ基因的克隆与表达 %A LIU Yun-Tao %A HAN Xue-Feng %A LUO Ze-Yu %A QIU Yu-Feng %A LONG Min-Nan %A HU Zhong %A
刘韫滔 %A 韩学凤 %A 罗泽宇 %A 邱玉峰 %A 龙敏南 %A 胡忠 %J 微生物学通报 %D 2012 %I %X Objective] The research focus on cloning endoglucanase I (egI) gene from Penicillium decumbens L-06 and expressing in Escherichia coli with high efficiency. Methods] egI gene was cloned from Penicillium decumbens L-06 by RT-PCR method. Recombinant plasmid pET32a-egI was constructed and was transformed into Escherichia coli rosetta(DE3). Recombinant protein with His-tag was expressed in E. coli rosetta(DE3) after induction with IPTG and then was purified with the Ni-NTA affinity chromatography. Results] As expected, the relative molecular mass was approximately 80kD after analyzed by SDS-PAGE and Western blotting. Hydrolysis activity of recombinant protein was assayed by cellulase activity staining and DNS method (2.56 IU/mL). Conclusion] The results achieve the purpose as constructing prokaryotic expression system and expressing egI gene. %K Penicillium decumbens L-06 %K endoglucanase I %K Cloning %K prokaryotic expression
斜卧青霉L-06 %K 内切葡聚糖酶Ⅰ %K 克隆 %K 原核表达 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=C8C3724E4E2B01E64F3FFB03EF6DE029&yid=99E9153A83D4CB11&vid=7C3A4C1EE6A45749&iid=94C357A881DFC066&sid=629F373584A92903&eid=0462D22862D5DA6F&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0