%0 Journal Article
%T THE SELECTION OF FLUOROACETATE-RESISTANT MUTANT FROM E. COLI MMR204 AND ITS INFLUENCE ON THE EXPRESSION OF HETEROLOGOUS GL-7ACA ACYLASE<br>大肠杆菌抗氟乙酸变株的选育及应用
%A Zhu Tongbo
%A Yang Yunliu
%A Jiao Ruishen
%A <br>朱彤波
%A 杨蕴刘
%A 焦瑞身
%J 微生物学报
%D 2000
%I 
%X In the cultivation of gene engineered strain of Escherichia coli on glucose medium, excretion and accumulation of acetic acid inhibit not only cell growth but also the the expression of heterologous protein. It is obvious that the desirable host strain maintaining acetate at a low level is one of the approaches to increase the production of recombinant protein. The present article deals with the selection of mutants of E. coli DP19, DP8, which grow on the medium containing pyruvate as the sole carbon source in the presence of 50mmol/L fluoroacetic acid. It is shown that mutant DP19 is defective in its phosphotransacetylase(PTA) activity and accumulates less acetate in the medium, while DP8 is defective in acetate kinase (ACK)and accumulates similar level of acetate comparing with its parent. Using pta mutant E. coli DP19 as host, the expression of GL-7ACA acylase gene on the recombinant plasmid pMR24 is improved, and the yield of enzyme activity in flask fermentation is about twice as much as its parent.
%K Fluoroacetic acid
%K Acetic acid
%K phosphotransacetylas (PTA)
%K Acetate kinase (ACK)
%K GL-7ACA acylase<br>氟乙酸
%K 乙酸
%K 磷酸转乙酰酶（PTA）
%K 乙酸激酶（ACK）
%K GL7ACA酰化酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=9DD9384BC24FA848&yid=9806D0D4EAA9BED3&vid=1371F55DA51B6E64&iid=CA4FD0336C81A37A&sid=8C83C265AD318E34&eid=DBF54A8E2A721A6D&journal_id=0001-6209&journal_name=微生物学报&referenced_num=6&reference_num=14