%0 Journal Article
%T Package of Bombyx mori Nuclear Polyhedrosis Virus with Heterogoneous Polyhedring Protein<br>异源多角体蛋白对家蚕核型多角体病毒粒子的包装
%A Ji Ping
%A Zha Xinmin
%A Zhang Guoying
%A Yu Jibin
%A Gu Wei
%A Shen Weide
%A <br>季平
%A 查新民
%A 张国英
%A 于继彬
%A 顾维
%A 沈卫德
%J 微生物学报
%D 2002
%I 
%X Cloning the segment of polyhedrin gene which was separated from AcMNPV genome DNA by PCR method into transfer vector pBacPAK8, we got recombinant transfer vector pOAc, then contransfecting BmN cells with the vector and linear virus Bm-BacPAK6, harvested recombinant virus hp-BmNPV which can produce polyhedrin but no blue plaques. Analysing of recombinant virus from polyhedrin, recombinant viral DNA and polyhedra, we confirmed that polyhedring of AcMNPV can be not only high-level expressed in BmN cells, But can recognize recombinant BmNPV DNA and assemble them to polyhedra. We observed that the pattern of restriction enzyme changed due to DNA recombination, the size of recombinant viral polyhedron (1.2-2.9 microns) was obviously smaller than those of wild-type BmNPV by electron microscopy.
%K Autographa californica nuclear polyhedrousis virus
%K Polyhedring
%K Bombyx mori nuclear polyhedrosis virus
%K Heterogeneous package<br>苜蓿尺蠖核型多角体病毒
%K 多角体蛋白
%K 家蚕核型多角体病毒
%K 异源包装
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=46DF13B53A79207C&yid=C3ACC247184A22C1&vid=ECE8E54D6034F642&iid=CA4FD0336C81A37A&sid=771152D1ADC1C0EB&eid=E514EE58E0E50ECF&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=11