%0 Journal Article
%T CLONING AND EXPRESSION OF D-ARABITOL DEHYDROGENASE GENE FROM ACETOBACTER SUBOXYDANS IN ESCHERICHIA COLI<br>弱氧化醋杆菌阿拉伯糖醇脱氢酶基因克隆及其在大肠杆菌中的表达
%A He Xiuping
%A Huai Wenhui
%A Guo Wenjie
%A Zhang Borun
%A <br>何秀萍
%A 怀文辉
%A 郭文洁
%A 张博润
%J 微生物学报
%D 2001
%I 
%X The partial genomic library of Acetobacter suboxydans was constructed using Yeast-E. coli shuttle plasmid YEp352 as vector. Two positive transformants, designated as DH5 alpha(pAD91) and DH5 alpha(pAD98), were obtained by screening the growth of transformants on the agar plate in which D-arabitol was used as the sole carbon source. The results of Southern blot and restriction endonuclease analysis showed that the two recombinants are identical. The insert is about 2.3 kb. Arabitol dehydrogenase activity assay indicated that the transformants could produce D-xylulose-forming D-arabitol dehydrogenase. Hence, the gene encoding D-arabitol dehydrogenase exists in the cloned DNA fragment.
%K Acetobacter suboxydans
%K D\|arabitol dehydrogenase gene
%K Gene cloning and expression<br>弱氧化醋杆菌
%K 阿拉伯糖醇脱氢酶基因
%K 克隆与表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=FFF4702E4D0DC14C&yid=14E7EF987E4155E6&vid=2001E0D53B7B80EC&iid=0B39A22176CE99FB&sid=FBA00558C57D9C11&eid=4B1FFFA116F7AE3B&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=7