%0 Journal Article
%T Study on hydrolysis technology of Lentinula edodes degraded by composite enzyme<br>复合酶法水解香菇工艺的研究
%A CHEN Hai-Qiang
%A HU Ru-Xiao
%A HUANG Xiao-Hui
%A PENG Yun-Xiang
%A WANG Chun-Hui
%A TAN Zhou-Jin
%A <br>陈海强
%A 胡汝晓
%A 黄晓辉
%A 彭运祥
%A 王春晖
%A 谭周进
%J 微生物学通报
%D 2012
%I 
%X Objective] In order to extract the functional components from Lentinula edodes efficiently for the further utilization of its edible value. Methods] Using Lentinula edodes as raw materials, the hydrolysis process conditions by composite enzyme including cellulose, papain and 5'-phosphodiester-ase were studied on the basis of single factor experiment and orthgonal test. Results] Firstly, it was hydrolyzed by 0.2% cellulose and 0.4% papain. The conditions were 55 °C, initial pH 5.5 and 3 h. Secondly, it was hydrolyzed by 0.2% 5'-phosphodiesterase. And the conditions were 70 °C, initial pH 7.0 and 2 h. Under these conditions, the hydrolysis degree was 39.48%, and the extracting rate of amino acids, 5'-nucleotide, polysaccharide were 10.25%, 0.768%, 8.67% respectively. Conclusion] The hydrolysate was rich in umami substances and other nutrients, and it could be used for condiment.
%K Lentinula edodes
%K Umami substance
%K Hydrolysis
%K Orthogonal test<br>香菇
%K 呈鲜味物质
%K 水解
%K 正交试验
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=4189763B19B8AD4580EA4207CF297B25&yid=99E9153A83D4CB11&vid=7C3A4C1EE6A45749&iid=CA4FD0336C81A37A&sid=CAEB9C70B35E6A8A&eid=B12528E4753014A0&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=13