%0 Journal Article
%T EXPRESSION OF THE ARTIFICIAL CECROPIN CM<br>中国家蚕抗菌肽人工合成类CMⅣ基因在甜菜夜蛾虫体中的表达
%A Zhao Donghong
%A Dai Zhuying
%A Zhou Kaiya
%A Zhang Linyuan
%A <br>赵东红
%A 戴祝英
%A 周开亚
%A 张林元
%J 微生物学报
%D 2001
%I 
%X The synthesized CMIV-like Gene was linked with the signal peptide gene of nature silkworm antibacterial peptide and was inserted into baculovirus expression vector pFastBac 1, construcing a recombinant transposing vector. The vector was transformed into DH10Bac competent E. coli cells. The recombinant Bacmid was obtained. The recombinant Bacmid was transfected into sf21 cells to get the recombinant virus. Laphygma exigua larvae were infected with the recombinant virus to express the antibacterial peptide. The hemolymph were tested to have antibacterial activity. The active antibacterial peptide was purified by acid polyacrylamide electrophoresis. The specific expression of mRNA of CMIV-like Gene was tested using Northern blotting.
%K 中国家蚕抗菌肽类CMⅣ基因
%K 昆虫杆状病毒表达系统
%K Bac-to-Bac策略
%K sf21细胞
%K 甜菜夜蛾幼虫
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=6B9209CC0D65CE30&yid=14E7EF987E4155E6&vid=2001E0D53B7B80EC&iid=B31275AF3241DB2D&sid=34D13857B558254E&eid=AF14A8B15FB15A64&journal_id=0001-6209&journal_name=微生物学报&referenced_num=5&reference_num=12